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. 2010 Sep 6;190(5):927–940. doi: 10.1083/jcb.201006105

Figure 6.

Figure 6.

CEP290 is dynamic and can incorporate into preassembled mutant or wild-type transition zones. (A) Wild-type gametic cells were mated with cep290 mutant gametic cells, and the fused, quadriflagellated cells were detergent-extracted, fixed, and processed for immunofluorescence at the indicated time points after the two cell types were mixed for mating. The quadriflagellated cytoskeletons were double labeled with antibodies to CEP290 and acetylated tubulin. Arrowheads mark the dimmer pair of transition zones. (B) Wild-type gametes were mated to cep290::CEP290-HA gametes and processed as in A except that CEP290-HA was visualized using anti-HA. Bars, 1 µm.