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. 2010 Sep 6;190(5):793–805. doi: 10.1083/jcb.201002055

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© 2010 Ray et al.

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Figure 1. — SIN activation arrests nuclear division and inhibits the interphase polarity machinery. (A) Cells of the indicated genotypes were grown at 25°C and then shifted to 36°C for 4 h, methanol fixed, stained with DAPI, and scored for number of nuclei (n). The experiment was done in triplicate and error bars denote SD. (B) Cells of the indicated genotypes were shifted to 36°C for 4 h and then stained with Alexa Fluor 488–phalloidin and DAPI to visualize actin and DNA, respectively. Dashed dividing lines separate individual images for montage presentations.