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. 2010 Sep 6;190(5):853–866. doi: 10.1083/jcb.201005117

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© 2010 O’Donohue et al.

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Figure 4. — Processing of the early cleavage site in the 5′-ETS of the primary transcript is still taking place upon depletion of RPS proteins. Pre-rRNAs uncleaved at site A’ were detected with probe 5′-ETSb, spanning nucleotides 301–324. This probe predominantly revealed the 45S pre-rRNA together with an extended form of the 30S pre-rRNA that we called 30S⁺¹. (A) The 30S⁺¹ species accumulated slightly on depletion of i-RPS proteins. However, the majority of the 30S RNA detected with probe 5′-ITS1 corresponds to a faster migrating band (overlay), indicating that it is processed at site A’. (B) Mild accumulation of 30S⁺¹ RNA was observed on depletion of some p-RPSs only, especially RPS18.