Figure 6.

Intracellular localization of the precursors to the 40S subunit in RPS-depleted cells. Pre-rRNAs of the 18S rRNA maturation pathway were detected by FISH with probes 5′-ITS1 (Cy3) and ETS1-18S (Cy5). (A–C) Control cells (A), representative examples of cells in which i-RPSs have been depleted (B), and cells in which p-RPSs have been knocked down (C). These FISH experiments were systematically performed in parallel with Northern blot analyses. Images were captured in identical conditions, and gray levels were scaled within the same lower and upper limits, except for RPS23-depleted cells for which the upper limit was twice that of the other images because of a much lower signal. The 5′-ITS1 labeling is displayed with a γ value of 1.5 to enhance the lowest gray levels. Bar, 10 µm.