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. 2010 Sep 6;190(5):835–852. doi: 10.1083/jcb.201002070

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© 2010 Petrovic et al.

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Figure 8. — EM analysis of MIS12C–SPC24–SPC25. (A) Negative-stain EM was performed on the recombinant MIS12C^NSL1-258–SPC24^57–197–SPC25^70–224 complex. (B) Class averages from negative-stain EM images shown in A. The arrowheads point to an element of density that is not present in the class averages of the MIS12C^NSL1-258 complex shown in Fig. 1 D and that likely represents the SPC24–SPC25 complex. Bars: (A) 10 nm; (B) 5 nm.