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. 2010 Sep 6;190(5):751–760. doi: 10.1083/jcb.201006029

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© 2010 Krahn et al.

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Figure 4. — Sdt colocalizes with Baz in the absence of Crb. (a) In a wild-type embryo at stage (st) 6, Crb is very weakly expressed, but Sdt and Baz colocalize in the apical portion of the lateral plasma membrane. (b) In the epidermis of a wild-type embryo at stage 8, Crb and Sdt colocalize slightly apical to Baz. (c) In the proctodeal invagination of a wild-type embryo at stage 8, Crb and Sdt colocalize slightly apical to Baz. (d and e) In embryos mutant for crb^8F105 (d) and crb^11A22 (e), Sdt and Baz colocalize in the absence of Crb. Note that the C-terminally truncated Crb^8F105 protein is diffusely localized in the epidermis, and thus, the fluorescence signal is below the detection level. (f) In a baz^815-8 mutant embryo derived from a germline clone (glc), Sdt is completely delocalized, whereas Dlg still localizes to the cortex. (g) In a sdt^K85 mutant embryo, Crb is completely delocalized, but Baz still localizes to apical spots at the membrane. See also Fig. S3. (a–g) Insets show an overview of the embryo from which the respective high magnification images were taken. Bars, 10 µm.