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. Author manuscript; available in PMC: 2010 Sep 8.
Published in final edited form as: Methods Mol Biol. 2008;447:369–380. doi: 10.1007/978-1-59745-242-7_24
Resolving gel solutions Stacking gel solution
Light-5% Heavy-12% or Heavy-16.5% 4%
Protogel 0.66 µL 1.60 µL 1.91 µL 0.60 µL
Water 1.97 µL 0.56 µL - 2.40 µL
3X Gel Buffer 1.34 µL 1.34 µL 1.16 µL 1.50 µL
Glycerol - 0.47 µL 0.40 µL -
10% AMPS 26.0 µL 26.0 µL 23.0 µL 70.0 µL
TEMED 4.0 µL 4.0 µL 3.50 µL 9.0 µL