TABLE I. Immunoreagents Used in This Study.
| Antibody specificity | Fluorescent label | Manufacturer |
|---|---|---|
| CD3 | FITC, PE, APC | eBioscience |
| PE-Cy5 | Pharmingen | |
| CD4 | FITC, PE | eBioscience |
| CD8 | FITC, PE, APC | |
| CD14 | eBioscience | |
| CD19 | PE | |
| CD25 | APC | Pharmingen |
| CD56 | FITC | eBioscience |
| CD83 | PE | Beckman Coulter |
| CD127 | PE | Pharmingen |
| HLA-DR | FITC | Biosource |
| PerCP | Pharmingen | |
| IgG | PE | Biosource |
| TGF-βa | PE | R&D Systems |
| IL-10a | PE | R&D Systems |
FITC, fluorescein isothiocyanate; PE, phycoerythrin; PerCP, peridinin chlorophyll protein; APC, allophycocyanin; Cy-5, cyanine 5.
a
Used for intracellular staining.