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Reactivity of the anti-human and anti-mouse CA XIV antisera with recombinant mouse CA XIV. An aliquot of 20 μg of total cell homogenate from CHO cells transfected with mouse CA XIV full-length cDNA was applied to each lane. Both antisera identified a major polypeptide of 54 kDa on Western blots. No signal was seen when preimmune serum was used in place of the first antibodies. MM, molecular mass.
