Figure 7.

Release of IFN-γ and IL-10 from the splenocytes of immunized mice. Three groups of mice (n = 3 in each case) were immunized (i.v.) with PBS, BTAA alone, or BTAA in combination with NLP weekly for 4 weeks. Seven days after the last injection, spleen cells from individual mice were isolated and cultured in RPMI 1640 medium in the presence of either PBS, BTAA alone or BTAA and NLP for 48 hours. The IFN-γ (A) and IL-10 (B) content of the culture supernatants was analyzed by ELISA using a commercially available kit. Each data point corresponds to the mean ± 1 SD. **,*Statistically different from PBS controls (P < 0.001 and P < 0.05, respectively).