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BORIS mRNA expression in DNMT deficient HCT116 cell lines. (A) Northern blot analysis of BORIS in the indicated cell lines. Total RNA from human adult testis tissue (Lane T) was run as a positive control. Ethidium bromide staining of the gel (lower panel) confirmed equivalent RNA loading. (B) Quantitative reverse transcriptase RT-PCR (qRT-PCR) analysis of BORIS in the indicated cell lines. BORIS expression was normalized to GAPDH. Error bars correspond to 1 SD.
