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RT-PCR analysis for PLAC1 expression in a subset of ovarian cancer specimens. PLAC1 mRNA expression yielded a 328 bp product. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) served as an endogenous control and yielded a 204 bp product. Shown is mRNA from placenta (lane 1). Lanes 2, 4, 6, and 7 are positive for PLAC1 mRNA expression whereas lanes 3, 8, and 9 are negative. Lane 5 has a negative GAPDH control and hence PLAC1 mRNA expression in this specimen is inconclusive.
