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NaPi2b/MX35 antigen biochemical characterization. Monoclonal antibody MX35 immune precipitations from metabolically-labeled SK-RC-18 cell lysates separated by SDS-PAGE under reducing conditions and visualized by autoradiography. (A) Enzymatic deglycosylation analysis of 35S-labeled immune complexes. (B) Enzymatic deglycosylation analysis of immune complexes derived from 3H-glucosamine labeled cells. (C) Acylation analysis of immune complexes from 3H-palmitate labeled cells.
