Figure 3.

UBXD4 is expressed in neuronal and non-neuronal tissues. a, RT-PCR experiments showed that UBXD4 mRNA is expressed in all the examined tissues including cortex, hippocampus, habenula, peripheral ganglia, and two different cell lines (HEK293 and PC12 cells). b, c, Analysis of UBXD4 at the protein level with anti-UBXD4 antibodies demonstrated that the prefrontal cortex, hippocampus, and habenula express UBXD4 endogenously. Due to the small size of the habenula, we used IP techniques to enrich for UBXD4 by pulling down UBXD4 with anti-UBXD4 antibodies immobilized on protein A/G beads followed by probing with anti-UBXD4 (see Materials and Methods for details).