Fig. 2.

Characterization of wrky34-1 and wrky-2 mutants, and developmental stage of the inflorescence at the time of cold treatment. (A) Exon and intron structure of the Arabidopsis WRKY34 gene, showing the locations of the T-DNA insertion sites in both mutants. Boxes indicate exons. (B) Relative WRKY34 RNA expression in mature pollen of the wrky34 mutants. Error bars indicate standard deviations of three independent determinations. Three independent experiments were shown by re-extracting RNA from other samples. Each experiment was also executed three times. (C) Flowers at stage 13 at the time of cold treatment are numbered ‘0’; flowers at more advanced stages are indicated by negative numbers.