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. 2010 Jul 19;61(14):3901–3914. doi: 10.1093/jxb/erq204

Fig. 4.

Fig. 4.

Phenotype of 35S::WRKY34 compared with the vector transgenic plant (control). (A) Inflorescence of a vector transgenic, 35S::WRKY34-1 and 35S::WRKY34-2 plants under normal growing conditions. (B) Relative WRKY34 RNA expression levels in mature pollen of 35S::WRKY34-1 and 35S::WRKY34-2 plants. (C) Fluorescence of mature pollen grains from vector transgenic plant, 35S::WRKY34-1 and 35S::WRKY34-2 plants stained with fluoroscein diacetate (FDA). (D) The proportion of viable pollen grains as determined by staining with FDA. (E) Elongated pollen tubes from vector transgenic, 35S::WRKY34-1 and 35S::WRKY34-2 plants stained with aniline blue. (F) Average number of elongated pollen tubes per stigma of 35S::WRKY34-1 and 35S::WRKY34-2 plants. Error bars indicate standard deviations of three independent biological samples.