Table 1.
Segregation analysis of progeny of self-fertilized heterozygous WRKY34/wrky34 plants with cold treatment
| Parent (self-fertilized) | Cold treatment | w34 | W34 | Ratio | χ2 |
| WRKY34/wrky34-1 | Control | 163 | 147 | 1.11:1 | 0.8 (P >0.05) |
| WRKY34/wrky34-1 | 24 h, 4oC | 153 | 115 | 1.33:1 | 5.4 (P <0.05) |
| WRKY34/wrky34-1 | 48 h, 4oC | 161 | 80 | 2.01:1 | 27.2 (P <0.01) |
| WRKY34/wrky34-2 | Control | 161 | 151 | 1.07:1 | 0.3 (P >0.05) |
| WRKY34/wrky34-2 | 24 h, 4oC | 149 | 114 | 1.31:1 | 4.7 (P <0.05) |
| WRKY34/wrky34-2 | 48 h, 4oC | 156 | 85 | 1.84:1 | 20.9 (P <0.01) |
The inheritance of wrky34 was analysed using PCR-based genotyping. w34 is the number of plants containing the wrky34 allele (plants with the WRKY34/wrky34 and wrky34/wrky34 genotypes), W34 is the the number of plants containing the WRKY34 allele (plants with the WRKY34/wrky34 and WRKY34/WRKY34 genotypes), Ratio is w34:W34. The χ2 test was used to compare the observed ratios with a predicted ratio of 1:1. The untreated greenhouse-grown plants were used as the control.