Table 2.
Analysis of the genetic transmission of WRKY34/wrky34 after Cold treatment
| Cold treatment | Parentage (female×male) | Wild type | Heterozygous | Ratio | χ2 |
| Control | WRKY34/wrky34-1×WT | 90 | 95 | 0.95:1 | 0.05 (P>0.05) |
| Control | WT×WRKY34/wrky34-1 | 101 | 99 | 1.02:1 | 0.01 (P>0.05) |
| 48 h, 4 oC | WRKY34/wrky34-1×WT | 77 | 84 | 0.92:1 | 0.12 (P>0.05) |
| 48 h, 4 oC | WT×WRKY34/wrky34-1 | 22 | 97 | 0.23:1 | 11.2 (P<0.01) |
| Control | WRKY34/wrky34-2×WT | 88 | 95 | 0.93:1 | 0.11 (P>0.05) |
| Control | WT×WRKY34/wrky34-2 | 94 | 98 | 0.96:1 | 0.04 P>0.05) |
| 48 h, 4 oC | WRKY34/wrky34-2×WT | 79 | 83 | 0.95:1 | 0.05 (P>0.05) |
| 48 h, 4 oC | WT×WRKY34/wrky34-2 | 33 | 107 | 0.31:1 | 8.11 (P<0.01) |
The inheritance of wrky34 was analysed by genotyping the F1 progeny of the specified crosses. Ratio is the observed wild-type (WT) to heterozygous frequencies. The WT parental plants were grown at 22 °C without cold treatment. The χ2 test was used to compare the observed ratios with a predicted ratio of 1:1. The untreated greenhouse-grown parental plants were used as the control.