Fig. 3.

Salt and osmotic sensitivity of wild-type, 35S-PeSCL7, scl7-1, and scl7-1/PeSCL7 plants. (A) Germination ratio of the wild-type, mutant, and transgenic plants in the absence or presence of NaCl (50 or 100 mM). Values are mean ±SE (n=3 experiments). (B) Water loss in wild-type, mutant, and transgenic plants. Detached leaves from 25-d-old plants grown on soil were incubated on a bench and the fresh weight (FW) was measured at the time intervals indicated. Water loss is expressed as the percentage of initial fresh weight of detached leaves. Values are mean ±SE (n=3 experiments). (C) Salt and osmotic effects on newly germinated seedling growth. Seeds of four different genotypes were germinated for 5 d on MS medium and MS medium containing 100 mM NaCl or 200 mM mannitol (Man), transferred to the same type of medium, and grown in a vertical position for an additional 1 d. Representative seedlings are shown. (D) Root elongation of four different genotypes under normal condition or salt and osmotic treatment (described above). Values are mean ±SE (n=3 experiments). (This figure is available in colour at JXB online.)