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In-gel assay of SOD activity in Arabidopsis wild-type and mutant lines. Protein extracts from leaves (80 μg of protein) were subjected to IEF (pH 3–7) and stained with NBT. SOD isoforms are indicated on the left and each band was quantified and expressed as a percentage of each isoform from the mutants in comparison with the wild type, which was arbitrarily considered 100%. The assay was repeated at least three times, and a representative gel is shown.
