Fig. 4.

Production of microspore-derived embryos (MDEs) from Brassica napus plants with altered BnSTM expression. Antisense lines (A, line 5 and 6) with lower BnSTM expression or sense lines (S, line 15, 101, 23, and 27) with increased BnSTM expression were utilized to generate MDEs. For each experiment 40 000 microspores were plated and embryo number was counted after 25 d in culture. Values ±SE (n=3) are expressed as a percentage of the WT. Asterisks above each bar indicate values which are significantly different from the WT value (P ≤0.05).