Table 1.
List of selected genes showing differential expression (fold change >4 and P ≤0.01) during progressive stages (1–3) of Arabidopsis somatic embryogenesis in the wild-type (WT) line and the 35S::BoSTM line
| WT |
35S::BoSTM |
|||||
| Stage 0–1 | Stage 1–2 | Stage 2–3 | Stage 0–1 | Stage 1–2 | Stage 2–3 | |
| Cytokinin | ||||||
| At3g23630 Adenylate isopentenyltransferase 7/IPST7 | – | – | – | +6.5 | – | – |
| At3g48100 Response regulator 5 (ARR5) | – | – | – | +40 | – | – |
| Ethylene | ||||||
| At5g03280 Ethylene-insensitive 2 (EIN2) | – | – | – | –7.6 | – | – |
| At5g25190 Ethylene-responsive element | –10.9 | – | – | –33.5 | +5.6 | – |
| At4g29100 Ethylene-responsive element | – | – | – | – | –12.9 | – |
| Gibberellins | ||||||
| At5g07200 Gibberellin 20-oxidase | +8.6 | – | – | +16.9 | – | – |
| At3g02885 Gibberellin-regulated protein 5 (GASA5) | – | – | – | –16.7 | – | – |
| Abscisic acid | ||||||
| At3g19290 ABA-responsive element-binding protein 2 (AREB2) | – | – | – | – | –7.4 | – |
| Auxin | ||||||
| At1g70940 Auxin transport protein (PIN3) | –6.0 | – | – | – | – | – |
| At1g19850 MONOPTEROS transcription factor | –9.8 | – | – | –50.1 | +8.7 | – |
| At2g33860 Auxin-responsive factor (ARF3) | – | –6.2 | – | –7.4 | – | – |
| At4g14560 IAA-induced protein 1 (IAA1) | –13.3 | +4.3 | – | +45.2 | +6.7 | – |
| At2g22670 IAA-induced protein 8 (IAA8) | – | – | – | +13.7 | –9.4 | – |
| At5g65670 IAA-induced protein 9 (IAA9) | – | – | – | +7.4 | –22.8 | – |
| At3g15540 IAA-induced protein 19 (IAA19) | – | +24.8 | – | – | +41.2 | – |
| At5g25890 IAA-induced protein 28 (IAA28) | – | – | – | +4.3 | –16.4 | – |
| Brassinosteroids | ||||||
| At4g39400 Brassinosteroid-insensitive 1 (BR1) | – | – | – | –6.7 | – | – |
| At3g50750 Brassinosteroid signalling-positive regulator | – | – | – | –7.1 | – | – |
| Signal transduction | ||||||
| At5g19280 KAPP | – | – | – | +4.9 | – | – |
| Transcription | ||||||
| At1g62630 SHOOTMERISTEMLESS (STM) | – | – | – | +5.2 | – | – |
| At2g17950 WUSCHEL (WUS) | – | – | – | +4.6 | – | – |
| At5g17430 Ovule development protein (homologue to BABY BOOM) | – | – | – | +7.5 | – | – |
| Post-transcription | ||||||
| At5g43810 PINHEAD/ZWILLE | – | – | – | +6.8 | – | – |
| At1g48410 ARGONAUTE 1 (AGO1) | – | – | – | +7.5 | – | – |
| Disease and defence | ||||||
| At4g35000 L-Ascorbate peroxidase 3 (APX3) | – | – | – | –6.3 | – | – |
| At5g21105 L-Ascorbate oxidase | –8.3 | – | – | –25.2 | – | – |
| At3g27820 Monodehydroascorbate reductase | – | – | – | –9.7 | – | – |
| At3g10920 Superoxide dismutase [Mn]-mitochondrial | – | – | – | – | –6.1 | – |
| At3g56350 Superoxide dismutase [Mn]-putative | –4.5 | +8.0 | – | – | +9.6 | – |
| At5g27380 Glutathione synthetase (GSH2) | – | – | – | – | –9.3 | – |
| At3g24170 Glutathione reductase (GR) | – | – | – | – | –6.7 | – |
| Metabolism | ||||||
| At2g26250 β-KetoacylCoA synthase (FIDDLEHEAD) | – | – | – | +13.7 | – | – |
| At3g17820 Glutamine synthetase (GS1) | – | – | – | – | –9.0 | – |
| At5g35630 Glutamine synthetase (GS2) | – | – | – | –8.0 | – | – |
| At5g49160 DNA (cytosine-5)-methyltransferase | – | – | – | –4.5 | – | – |
| At5g25480 DNA (cytosine-5)-methyltransferase | – | – | – | –9.2 | – | – |
Positive values indicate an up-regulation of gene expression between two adjacent stages.