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. 2010 Sep 8;5(9):e12626. doi: 10.1371/journal.pone.0012626

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Ashworth et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Glomeruli recovered from patients with glomerular diseases, FSGS, MCD and MGN, were probed with the rabbit primary antibody to p-cofilin and goat primary antibody to podocalyxin (PDX) and visualized using an Alexa488 labelled antibody raised against goat (green) and a Cy3-labelled secondary antibody against rabbit (red). Podocytes in these diseased glomerular tissues demonstrated an increased incidence of phosphorylated cofilin-1 in their nuclei (merged images ±DAPI). In contrast p-cofilin of normal control patients revealed no glomerular staining indicating that the cofilin-1 expressed in the glomerulus under normal conditions is active and under disease-conditions is inactivated.