Table 2.
ARPE-19 Cells Produce Laminin RNAs
| Laminin Chain | Primer (5′–3′) | ARPE-19 Cells | Controls | |||
|---|---|---|---|---|---|---|
| Forward | Reverse | Skin | Ret | RMC | ||
| α1 | TGTAGATGGCAAGGTCTTATTTCA | CTCAGGCAGTTCTGTTTGATGT | + | ND | + | + |
| α2 | CAAAGAYATTGAAATTTCAAG | GAGTTAATAACAAGRTTCCA | − | + | + | − |
| α3 | CTCATGGTGAGATACAAACT | TTGAAAGGTCTGAAACCCAAA | + | + | ND | ND |
| α4 | GGATGCGCCTTCATGGG | GTCAGGCTGTGGGACAGGA | − | ND | + | + |
| α5 | GAAGCTGGCTCTTGTCATCC | GCATAATCCAGGCCAAAGAA | + | + | + | + |
| β1 | TAACGAGGTGGAGTCCGGTTA | AAGGCCCGTCTGGTGAATCAA | + | ND | ND | + |
| β2 | CTTCGCTTGGGCCTACTTCT | GGATGGTGACCATCGGAACA | + | ND | + | + |
| β3 | AATGTAGTGGGCCCCAAATG | CTCCTTCTTGCTTCGGTAC | + | + | ND | + |
| γ1 | ACGGCTACTTTGGAGACCCT | GTCCAAACCCAAAGTGGTTG | + | ND | ND | ND |
| γ2 | ACCCCCGCAGCTGCAAGCCRTGT | GCAAGCTCGACACTTGTCTGCT | + | + | + | + |
| γ3 | TACGGTAACGCCTTCTCAGG | CAAGTGGGTGACATTTGCAG | − | ND | + | ND |
| γ3* | TATGGCAACCCTTTCGCGGG | CCAGTGGGTGACACTTGCAG | − | ND | ND | ND |
The human RPE cell line ARPE-19 and several other cell types were analyzed for expression of RNA encoding all 11 laminin chains. ND, not determined.
*
“Humanized” primers.