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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Ann Neurol. 2010 Aug;68(2):151–161. doi: 10.1002/ana.22104

Fig. 1.

Fig. 1

Western blotting of brain homogenates after limited digestion with PK shows strain typing of sCJD (A, B) and vCJD (C, D) prions. (A, B) sCJD prions in the brain of human sCJD case HU178 (lanes 1) retain type 1 PrPSc after passage in Tg1014 mice (A, lane 2) and Tg22372 mice (B, lane 2). (C, D) vCJD prions in the brain of human vCJD case RU96/45 before (lanes 1) and after passage in Tg1014 (C, lanes 2–11) and Tg22372 (D, lanes 2–10) mice. The incubation period, in days, for each mouse is listed above the respective lane. vCJD prions retained type 2 PrPSc in Tg mice with longer incubation periods, but changed to type 1 PrPSc in Tg mice with shorter incubation periods. Tg mice with intermediate incubation periods (190 d in C, 279 d in D) showed a mixture of type 1 and type 2 PrPSc. Molecular weight markers represent protein standards of 20 and 30 kilodaltons.