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. Author manuscript; available in PMC: 2011 Sep 8.
Published in final edited form as: Cell Metab. 2010 Sep 8;12(3):224–236. doi: 10.1016/j.cmet.2010.06.009

Fig. 5. SIRT6 regulates the expression of genes related to glycolysis and lipid metabolism.

Fig. 5

(A,B) mRNA levels of GK and LPK in the liver of WT and MT mice under fed Ad Libitum (AL) or 24 hours starvation (S) detected by using Real-Time RT-PCR. (C) Protein expression of genes involved in glycolysis and lipid metabolism. (D) GK enzymatic activity in the liver of WT and MT mice. (E-G) mRNA levels of FAT and FATP (E,F) and several genes involved in TG synthesis (G). Mice used in panels (A,F,G) were 8–9 months old, and in panels (B-E) were 2–3 months old. At least 5 pairs of mice were used for each experiment. (H) De Novo lipogenesis activity in the primary hepatocytes under fed or fasted condition. (I,J) Absence of SIRT6 decreased the expression levels of genes involved in β-oxidation revealed by Real-Time RT-PCR (I) and fatty acid β-oxidation revealed by enzymatic activity (J). (K) Gene expression in primary hepatocytes upon acute knockdown of SIRT6 for 36 hours revealed by Real-Time PCR. Abbreviation of genes that is not mentioned in the text: ACC: acetyl-CoA carboxylase; Elovl6: long-chain elongase; FAS: Fatty acid synthase; GPAT: mitochondrial glycerol 3-phosphate acyltransferase; SCD1: stearoyl-CoA desaturase-1.