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Internal acidification in 100 mM Rb solutions reversibly closed R128Y with a pKa of 6.7 ± 0.03 (black). The same acidification in 100 mM external K closed R128Y with a pKa of 6.8 ± 0.03 (red), similar to the pKa of 6.7 ± 0.02 for wt-Kir1.1b. however, R128Y recovered very slowly during realkalization in 100 mM K (dashed red line).
