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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: J Am Chem Soc. 2010 Sep 15;132(36):12534–12536. doi: 10.1021/ja1050814

Table 1.

Kinetic parameters of MdpB2 towards aromatic substrates featuring 1,2-hydroxy acid functionality (highlighted in red).

graphic file with name nihms230312u1.jpg
Substrate KM (μM) kcat (min−1) rel kcat/KM
y 0.041 ± 0.005 2.32 ± 0.06 1
4 2.1 ± 0.3 0.59 ± 0.02 0.0049
8 0.23 ± 0.05 0.90 ± 0.03 0.069
9 1.2 ± 0.2 0.86 ± 0.04 0.013
10 ≥0.0042a
11 ≥0.0013a
a

Values represent specific activities at 100 μM substrate concentration.