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. 2010 May 5;16(9-10):359–371. doi: 10.2119/molmed.2009.00168

Figure 8.

Figure 8

Effect of PRAS40 knockdown on autophagy. Myoblasts were transfected with either control (shScramble) shRNA or shRNA targeting PRAS40, and cell lysates were collected for Western blotting. (A) Representative Western blots of whole cell lysates probed using antibodies against total Atg 7, Beclin 1, LC3B and PRAS40 are shown. Nutrient-starved myoblasts treated with Hanks’ balanced salt solution served as the positive control. (B) Quantification of Western blot data. Values are means ± SE; n = 10 per group. *P < 0.05 compared with time-matched scramble control values.