Fig. 4.

Survival of strains KZR-5 and 715 at 4°C (A) and virulence on tomato plants (B). A Culturability (the average of three replicates) of strains KZR-5 and 715 upon exposure to 4°C at t = 1 (bar 1) and t = 84 days (bar 2). - - - - - -, limit of detection (10 CFU/ml). B Average wilting of 4–5 week old tomato plants at 7, 14 and 21 days post inoculation. Plants were inoculated 25 ml of cell suspension containing 10⁺⁵ CFU/ml, incubated in the greenhouse at 26°C (day, 14 h)/21°C (night, 10 h), and disease development in the plants was scored at regular intervals over time using a disease matrix ranging from 0, no wilting symptoms, to 4, all leaves wilted. The average wilting index was determined as wilting symptoms of all plants/number of plants