Figure 6.

Intracellular diffusion of free Cy5. T-REx-293 cells expressing green-fluorescent protein fusion proteins of dihydrofolate reductase (DHFR-GFP) were electroporated with Cy5. (A) Characteristic autocorrelation functions for DHFR-GFP (dashed line, green) and Cy5 (dotted line, red) and the cross-correlation function (solid line). The fits of the autocorrelation functions are included. Cy5 was fitted with a model containing two diffusing components. (B) Characteristic cross-correlation function at a larger scale. (C,D) Dot plots of the relative contribution to the autocorrelation amplitude versus diffusional autocorrelation time τ_D for Cy5 with (C) the fast component variable and (D) the fast component fixed to 128 µs with averages (cross) and standard deviations.