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Effect of sildenafil on carbachol-induced human detrusor contraction. Concentration–response curves to sildenafil or vehicle were performed on human detrusor strips, pre-contracted with carbachol (1 µM). The data are mean ± SEM of 20 bladders. ###P < 0.001, two-way anova followed by a modified Student's t-test with the Bonferroni adjustment for multiple comparisons, because of interaction in the two-way anova analysis ***P < 0.001.
