Figure 3.

Diverse effects of parthenolide (PN) and/or cisplatin (cisPt) on cell cycle progression in A375, 1205Lu, WM793 cells and normal melanocyte (NHEM) cultures. (A) Distribution of the adherent cells through the cell-cycle phases was analysed by flow cytometry in adherent cells treated with drugs or vehicle for 24 h. Representative histograms and data from three independent experiments are shown. To calculate percentages of the cells in each fraction ModFit LT 3.0 software was used. For the parthenolide-treated 1205Lu cells, it was not possible to obtain valid data because of the high percentage of cells in the hypodiploid fraction. Therefore, parthenolide at lower concentration (3 µM) was used either alone or in combination with 5 µM cisplatin (B). (C) Parthenolide at 12 µM did not affect distribution of normal melanocytes (NHEM) in cell cycle and did not increase their accumulation in subG₁ phase. NHEM, neonatal human epidermal melanocytes.