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HPLC profile of isolated RCSL 0520. Extracts of Eulophia ochreata were purified, using activity-guided chromatographic fractionation on silica gel. On subsequent HPLC, the most active fraction showed a single peak (fraction 5) with a retention time of 17.3 min, exhibiting >98% of the absorbance eluted and was RSCL-0520 (∼99% pure).
