RSCL-0520 suppressed LPS-induced TNF-α output, in a concentration-dependent manner, in different cell lines. Human monocytic cells (THP-1) (A), human PBMCs (B) and mouse macrophage cells (RAW264.7) (C) were pretreated with the indicated concentrations of RSCL-0520 followed by LPS stimulation for 24 h. Culture supernatants were collected and assayed for TNF-α by elisa. Data are expressed as means ± SEM of three independent experiments (***P < 0.001, **P < 0.001, *P < 0.05; LPS treated vs. RSCL-0520 treated).