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. 2010 Jul;160(5):1158–1170. doi: 10.1111/j.1476-5381.2010.00780.x

Figure 7.

Figure 7

RSCL-0520 inhibits IL-8, IL-1β and COX-2 protein expression in THP-1 cells. THP-1 cells were pretreated with the indicated concentrations of RSCL-0520, and then stimulated with LPS for 24 h. Culture supernatants were collected and assayed for IL-8 (A) and IL-1β (B) by elisa. Data are expressed as means ± SEM of three independent experiments (***P < 0.001; LPS treated vs. RSCL-0520 treated). In Figure 7C, cell lysates were prepared from serum-starved THP-1 cells pretreated with 50 µM RSCL-0520 followed by LPS stimulation for the indicated time-points. Immunoblotting was carried out to determine the COX-2 protein levels. The same blot was probed with tubulin antibody to ensure equal loading.