Skip to main content
. 2010 Jul;160(5):1158–1170. doi: 10.1111/j.1476-5381.2010.00780.x

Figure 8.

Figure 8

RSCL-0520 blocks activation of NEMO and IκBα degradation. Serum- starved THP-1 cells were pre-incubated with 50 µM RSCL-0520 for 1 h, and then stimulated with LPS for the indicated time. Total protein was isolated from the treated cells, and an equal amount of protein from each sample was used for immunoblots to determine protein levels of NEMO and IκB-α. The blot was stripped and reprobed with an anti-ERK-1/2 antibody to ensure equal loading. Quantification of NEMO and IκB-α bands was normalized by ERK-1/2, and data presented are from three independent experiments. ***P < 0.001; **P < 0.01; LPS treated-15, 30, 60 min versus RSCL-0520 + LPS treated-15, 30 and 60, respectively.