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. 2010 Jul;160(5):1158–1170. doi: 10.1111/j.1476-5381.2010.00780.x

Figure 9.

Figure 9

RSCL-0520 blocks phosphorylation and nuclear translocation of NF-κB. Cells stimulated with LPS for 1 h were processed for FACS using an NF-κB-specific antibody tagged with Alexa-Fluor-488. The results shown (A) are representative of three separate experiments. Adjacent graph shows MFI data from three experiments (**P < 0.01; LPS treated vs. RSCL-0520 + LPS treated). Treatment of cells with RSCL-0520 alone did not show any alteration in protein levels (data not shown). In B, the nuclear fractions were obtained from LPS-stimulated THP-1 cells at the indicated times, and processed for immunoblots using an NF-κB-specific antibody, as in A. Quantification of p65NF-κB bands was normalized by tubulin. ***P < 0.001, **P < 0.01; LPS-treated vs. RSCL-0520 + LPS at 30, 60 and 120 min. All the immuno blot results shown are representative of three separate experiments.