Skip to main content
. 2010 Jul;160(5):1195–1211. doi: 10.1111/j.1476-5381.2010.00762.x

Figure 7.

Figure 7

Effect of BAPTA-AM, W7 and Gö6976 on migration and invasion in HT-1080 cells. (A) Cell monolayers were scratched with a pipette tip and then treated with BAPTA-AM (10 µM), W7 (40 µM) and Gö6976 (a calcium-dependent PKC inhibitor; 1 µM) for 24 h. Migrating cells were photographed under phase contrast microscopy. Each figure is representative of at least three others. Data are expressed as the means ± SD of triplicate experiments. *Significantly different from control (P < 0.01). (B) Cells were pretreated with BAPTA-AM (10 µM), W7 (40 µM) and Gö6976 (1 µM) followed by PMA (30 nM) treatment for 24 h. After 24 h, cells on the bottom side of the filter were counted. Data are expressed as the means ± SD of triplicate experiments. *Significantly different from PMA treatment only (P < 0.01).