Figure 5.
Effects of CBD (50 µg·kg−1) and I/R on the percentage of mast cells degranulated in the rat myocardium. Mast cell degranulation was measured as the percentage of the total number of mast cells present that had undergone degranulation and is expressed as the mean ± SEM (n = 6–9). The percentage incidence of mast cell degranulation was determined at a magnification of ×400 and encompassed an entire cross-section of ventricular tissue. Both sham-operated and I/R animals were treated with a bolus dose of either vehicle or CBD. The effect of I/R alone on mast cell degranulation was determined via a comparison of vehicle-treated I/R animals with vehicle-treated sham-operated animals. ***P < 0.001 versus vehicle sham-operated. CBD, (-)-cannabidiol; CBD-PI, pre-ischaemia CBD-treated group; CBD-PR, pre-reperfusion CBD-treated group; I/R, ischaemia/reperfusion.