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. 2010 Jun 9;95(9):E9–E17. doi: 10.1210/jc.2010-0460

Figure 4.

Figure 4

A, Bar graph showing the concentration-dependent inhibitory effects of resveratrol (Res; 0.1–50 μmol/liter) and CYP450 inhibitor ABT (10 μmol/liter) on the conversion of estradiol (25 μmol/liter) to 2-hydroxyestradiol (2-OHE) in microsomes. B, Concentration-dependent inhibitory effects of resveratrol on 2-methoxyestradiol (2-ME) formation in VSMCs incubated with microsomal extracts (1 mg/ml) that were incubated for 2 h with estradiol (25 μmol/liter). C, Inhibitory effects of 10 μmol/liter of COMT inhibitor OR486 and resveratrol (Res; 10 and 100 μmol/liter) on the conversion of 2-hydroxyestradiol (0.25 μmol/liter) to 2-methoxyestradiol (2-ME) formation in VSMCs. D, Inhibitory effects of resveratrol (1–100 nmol/liter) and ABT (10 μmol/liter) on the conversion of [2-3H]estradiol to 2-hydroxyestradiol and 3H2O in VSMCs incubated for 12 h. Values represent mean ± sem from at least three independent experiments each conducted in triplicate. *, P < 0.05 vs. 2-methoxyestradiol or 2-hydroxyestradiol formation in absence of inhibitors.