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Immunoblot analysis of the antiserum directed at the catalytic beta subunit of the F-ATPase-1 gene cluster found in the K. stuttgartiensis genome. Lane 1: Marker (PageRuler™ Prestained Protein Ladder Plus, Fermentas); lane 2: incubation with anti-F-ATPase-1; lane 3: incubation with the pre-immune serum; lane 4: incubation with only the secondary antibody. Arrow: expected target size (52 kDa).
