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. 2010 Jul 1;59(3):493–503. doi: 10.1111/j.1574-695X.2010.00710.x

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© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Fig. 4 — Representative DIGE gel from mono- and dual-species biofilms. Whole-cell lysates, enriched in the cytoplasmic fraction, were obtained from 24-h biofilms. Proteins (100 μg) were differentially labeled with CyDye: Candida albicans labeled with Cy2 (blue), Staphylococcus aureus labeled with Cy3 (green), dual-species biofilm proteins labeled with Cy5 (red). Proteins were focused in the first dimension on pH 3–10 IEF strips and resolved in the second dimension on 12.5% polyacrylamide gels. (a) Representative gel from staphylococcal–yeast biofilms. (b) Representative gel from staphylococcal–hyphal biofilms.