Table 1.
Proteins upregulated in the dual-species biofilm
| Spot | MW (Da) | pI | Organism | Identity | Protein name | Peptide matches | Protein score confidence interval (%) | Accession number | Function |
|---|---|---|---|---|---|---|---|---|---|
| (A) Proteins upregulated in staphylococcal–yeast biofilms | |||||||||
| 1A | 84624.8 | 5.96 | C. albicans | Putative mitochondrial aconitate hydratase | Aco1p | 19 | 100 | 68479387 | Carbohydrate metabolism; tricarboxylic acid cycle |
| 14A | 21481.5 | 5.15 | C. albicans | Similar to heat shock protein 5 | Similar to Hsp5 | 9 | 100 | 68469633 | Cellular stress response; protein folding |
| 15A | 91795.2 | 6.35 | C. albicans | Heat shock protein 78 | Hsp78p | 16 | 100 | 31076745 | Cellular stress response; protein folding |
| 16A | 26893.9 | 5.74 | C. albicans | Triosephosphate isomerase | Tpi1p | 10 | 100 | 7270988 | Glycolysis; gluconeogenesis; fatty acid biosynthesis |
| 17A | 21960.3 | 4.98 | C. albicans | Thioredoxin peroxidase | Tsa1p | 7 | 99.99 | 68479826 | Cellular stress response; antioxidant |
| 18A | 49188.7 | 7.36 | C. albicans | Metal-binding activator 1 | Mac1p | 6 | 85.35 | 68471167 | Copper-binding transcriptional regulator; cellular stress response |
| 19A | 95340.7 | 5.68 | S. aureus | Alcohol dehydrogenase, iron containing | Adh | 15 | 100 | 57651152 | Carbon utilization; alcohol metabolism |
| 20A | 95397.8 | 5.73 | S. aureus | Putative aldehyde-alcohol dehydrogenase | AdhE | 16 | 100 | 49482391 | Carbon utilization; putative peroxide scavenger |
| 21A | 56138.6 | 6.02 | S. aureus | Probable malate:quinone oxidoreductase | Mqo1 | 21 | 100 | 82752186 | Carbohydrate metabolism; tricarboxylic acid cycle |
| 22A | 37820.9 | 5.14 | S. aureus | Ornithine carbamoyltransferase | ArgF | 12 | 100 | 49484831 | Amino acid biosynthesis |
| 23A | 35194.1 | 4.65 | S. aureus | Pyruvate dehydrogenase complex E1 component β | PdhB | 11 | 100 | 57651703 | Glycolysis; oxidoreductase |
| 24A | 35539.3 | 5.36 | S. aureus | Carbamate kinase | ArcC1 | 17 | 100 | 49484829 | l-Arginine degradation |
| 25A | 28737.4 | 5.87 | S. aureus | Transcriptional repressor CodY | CodY | 8 | 99.97 | 15924245 | Decreased hemolysin, biofilm, and quorum-sensing function |
| 26A | 23092.2 | 6.08 | S. aureus | Uracil phosphoribosyl transferase | Upp | 10 | 100 | 15925102 | Pyrimidine metabolism |
| 27A | 63331.2 | 5.2 | S. aureus | Pyruvate kinase | Pyk | 27 | 100 | 49483939 | Carbohydrate metabolism; glycolysis |
| (B) Proteins upregulated in staphylococcal–hyphal biofilms | |||||||||
| 1B | 93865.5 | 6.07 | C. albicans | Translation elongation factor 2 | Eft2p | 4 | 100 | 68481380 | Protein synthesis |
| 8B | 35924.7 | 6.61 | C. albicans | Glyceraldehyde 3 phosphate dehydrogenase | Thd1p | 15 | 100 | 68472227 | Carbohydrate metabolism; glycolysis |
| 11B | 33026.2 | 5.4 | S. aureus | Cysteine synthase | CysK | 4 | 100 | 82750220 | Cysteine biosynthesis |
| 3B | 29543.3 | 5 | S. aureus | l-Lactate dehydrogenase | Ldh1 | 9 | 100 | 87161566 | Growth during nitrosative stress |
| 12B | 40322.9 | 5.2 | S. aureus | Alanine dehydrogenase 1 | Ald1 | 15 | 100 | 21283057 | Cell wall synthesis; oxidation reduction |
| (C) Proteins upregulated in both biofilm conditions | |||||||||
| 9A,B | 21481.5 | 5.79 | C. albicans | Similar to phosphoglycerate mutase | Gpm1p | 16 | 100 | 68469783 | Carbohydrate metabolism; glycolysis |
| 10A,B | 17677.9 | 7.74 | C. albicans | Cyclophilin type peptidyl-prolyl cis–trans isomerase | Cyp1p | 4 | 100 | 68469052 | Protein folding; cellular stress response |
| 13A,B | 55751.8 | 6.54 | C. albicans | Pyruvate kinase | Pyk1p | 16 | 100 | 68482226 | Carbohydrate metabolism; glycolysis |
| 2A,B | 36423.8 | 5.34 | S. aureus | Alcohol dehydrogenase | Adh | 12 | 100 | 21282297 | Carbon utilization; alcohol metabolism |
| 5A,B | 29434.3 | 5.34 | S. aureus | 30s ribosomal protein S2 | RpsB | 6 | 100 | 57651825 | Protein synthesis; stress response |
| 6A,B | 18520.5 | 5.6 | S. aureus | Similar to universal stress protein family | Similar to UspA1 | 7 | 95.7 | 15924700 | Cellular stress response |
| 4A,B | 37381.6 | 6.08 | S. aureus | Threonine dehydratase | IlvA | 16 | 100 | 147733998 | Amino acid metabolism |