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. Author manuscript; available in PMC: 2010 Sep 9.
Published in final edited form as: Mol Cell. 2007 Jul 6;27(1):121–133. doi: 10.1016/j.molcel.2007.05.026

Figure 1. Differential Expression of p21 during Stimulus-Specific Responses to p53 Activation.

Figure 1

(A) Cell-cycle profiles of HCT116 cells 24 hr posttreatment with UVC (25 J/m2) or Nutlin 3 (10 µM) were determined by FACS analysis of DNA content. Pie charts show the percentage of cells in each phase of the cell cycle.

(B) Western blot analysis of HCT116+/+ cells at the indicated times following treatment with UVC or Nutlin 3 showing accumulation of total p53, phospho-Ser15 p53 (S15P-p53), and p21.

(C) Real-time PCR analysis of p21 and Btg2 mRNA induction following UVC and Nutlin 3 treatment expressed as fold induction over untreated cells after normalization to 18S rRNA levels. Error bars represent the standard deviation.