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. Author manuscript; available in PMC: 2010 Sep 9.
Published in final edited form as: Mol Cell. 2007 Jul 6;27(1):121–133. doi: 10.1016/j.molcel.2007.05.026

Figure 2. Stimulus-Specific RNAP II Activation at the p21 Locus.

Figure 2

(A) Schematic of the p21 locus indicating the two p53 binding sites (high-affinity p53BS1 and low-affinity p53BS2) and overall gene structure. Amplicons used in real-time PCR quantification of ChIP-enriched DNA are named according to their relative distance (bp) to the transcription start site.

(B) ChIP analysis was carried out using protein extracts of untreated and UVC- or Nutlin-treated cells (8 hr) using antibodies against total p53 (p53), phospho-Ser15 p53 (S15P-p53), acetylated histone H4 (Ac-H4), acetylated histone H3 (Ac-H3[K9]), total RNAP II, and phosphorylated Ser5 and Ser2 forms of RNAP II (S5P-CTD and S2P-CTD). Numbers of PCR and ChIP replicates are indicated. Standard deviation is below 20%.