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. Author manuscript; available in PMC: 2010 Sep 9.
Published in final edited form as: Mol Cell. 2007 Jul 6;27(1):121–133. doi: 10.1016/j.molcel.2007.05.026

Figure 7. CDK8 Acts as a Positive Regulator of p53-Dependent p21 and Hdm2 Expression.

Figure 7

(A) Western blot analysis of untreated and Nutlin 3-treated (16 hr) HCT116+/+ cells following knockdown of CDK8.

(B) Real-time PCR analysis of p21 and Hdm2 mRNA induction in cells transfected with control of CDK8-specific siRNA. Data is expressed as fold induction over untreated cells and normalized to 18S ribosomal RNA levels. Error bars represent the standard deviation.

(C) p21 and Hdm2 reporter assays in Saos-2 cells transfected with p53, CDK8, and cyclin C expression vectors as noted. Normalized values are shown as fold induction relative to empty vector alone. Error bars represent the standard deviation.