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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Curr Protoc Cell Biol. 2009 Dec;CHAPTER:Unit–10.15. doi: 10.1002/0471143030.cb1015s45

Figure 13.

Figure 13

Reflected light images while trying to find the PVA thin film. A and B) Images were taken while scanning in the Z-plane. The bright linear areas indicate passing by the glass/PVA thin film (arrows). C) Once found, the field of view (FOV) should appear equally (uniformly) bright if the surface is level.