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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Curr Protoc Cell Biol. 2009 Dec;CHAPTER:Unit–10.15. doi: 10.1002/0471143030.cb1015s45

Figure 14.

Figure 14

Dish leveling A) Reflected light image of a dish that is slightly tilted down on the right as shown by the increase in brightness as you focus up to the glass surface. Below the image is a cartoon side view representing the glass (blue) with respect to the horizon (black line). B) Image of the confocal stage. Inset shows a magnified view (white box) of one of four adjustment screws in the stage plate. The other adjustment screws are indicated by asterisks. C) Simplified schematic of the tilted dish in panel A and how to correct the tilt by screwing in both right-side adjustment screws, until an in-focus evenly illuminated FOV is attained (right-side of arrow).