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. Author manuscript; available in PMC: 2010 Sep 9.
Published in final edited form as: Circ Res. 2009 Jun 18;105(2):201–208. doi: 10.1161/CIRCRESAHA.109.196790

Figure 5. Knockdown of AGM results in a severe impairment in vascular patterning.

Figure 5

A) Total RNA extracted from zebrafish injected with SPL-MO was assessed for AGM knockdown by RT-PCR and qPCR. SPL-MO resulted in a decrease of AGM mRNA to 5% of control. 24 hpf. B) Lateral views of 39 hpf control and AGM ATG-MO-injected embryos (ATGMO, 5 ng). The morphant phenotype manifests in pericardial edema and defects in yolk-extension, circulation, brain, and melanophores. 10 ng of ATG-MO produces a more severe phenotype. SPL-MO yielded similar phenotypes. C) 2.5 ng of ATG-MO injected in fli1:EGFP transgenic zebrafish embryos results in ISV defects. Axial vessels appear correctly specified. 48hpf. D) fli1:EGFP fish treated with 10ng ATG-MO or control MO demonstrating defect in ISV patterning. 56 hpf. E) 20ng of SPL-MO results in similar defects in ISV patterning. 48hpf.